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. 2021 Apr 6;12(4):368. doi: 10.1038/s41419-021-03645-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A The LC3 and SQSTM1 protein levels in MKN-45/MALAT1, MGC-803/MALAT1, and their parental cells were determined by western blot assay; B, C The LC3 and SQSTM1 protein levels in MKN-45/MALAT1 and MGC-803/MALAT1 cells with 3-MA (10 mM) and baf-A1 (10 mM) were determined by western blot assay; D, E mRFP-GFP-LC3 distribution in MKN-45/MALAT1, MGC-803/MALAT1, and their parental cells were analysed by fluorescence microscopy (MKN-45/MALAT1 vs MKN-45/NC: 50.3 ± 4.5 vs 11.3 ± 2.6; MGC-803/MALAT1 vs MGC-803/NC: 28.3 ± 6.2 vs 2.6 ± 1.6, P < 0.01); F, G The number of autophagic vesicles was increased in MKN-45/MALAT1 and MGC-803/MALAT1 group as seen by TEM.