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. 2021 Apr 6;10(4):32. doi: 10.1038/s41389-021-00321-2

Fig. 2. Endogenous CARD10 cleavage in A549 cells and primary HuVEC cells.

Fig. 2

A A549 cells were treated with 5 µM of the proteasome inhibitor MG-132 and stimulated for 2 h with P/I ± 1 µM of MLT-748. CARD10 immunoprecipitation was performed and CARD10 expression assessed by Western blotting together with the two MALT1 substrates HOIL1 and RelB (N = 3). B HuVECs were treated and analyzed as done in (A) for A549 (N = 2). C CARD10 mRNA levels obtained from microarray analysis with probe 210027 (top panel). CARD10 immunoblot performed on full lysates obtained from A549-WT, -KO, and -KI cells (bottom panel). D A549-WT and KI cell lines were treated for 2 h with P/I (50 µg/ml /1 μM) or TNF (10 ng/ml) as non-CBM activation control in presence of 5 μM of MG-132. Relative quantitative PCR measurement of CXCL1 mRNA; Mean ± SD of triplicates (N = 2). RelB cleavage was then assessed by Western blot (bottom panel).