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Target residues for mutations to stabilise the de novo protein WA20 in the present study. The target amino acid residues are shown as sticks. Chains A and B of the crystal structure of WA20 (PDB: 3VJF)16 are shown in magenta and cyan, respectively. The images were created using open-
source PyMOL, version 2.4 (https://github.com/schrodinger/pymol-open-source).
