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Denaturation midpoint temperatures (Tm) of the SUWA mutants.
| Sample | Tm (°C) | ΔTm (°C) |
|---|---|---|
| SUWA | 124.7 ± 0.1 | – |
| SUWA_N22A | 128.5 ± 0.1 | 3.8 |
| SUWA_N22E | 125.8 ± 0.1 | 1.1 |
| SUWA_H86K | 125.6 ± 0.3 | 0.9 |
| SUWA_N22A/H86K | 128.5 ± 0.5 | 3.8 |
| SUWA_N22E/H86K (ROSA) | 129.0 ± 0.1 | 4.3 |
