Fig. 3. Experimental setup for each treatment (dsαCOP at 0.5, 2.5 and 5 µg/µL, and dsGFP at 5 µg/µL) for both short-term (3 days) and chronic (17 days) dsRNA feeding in Brassicogethes aeneus RNAi assays.

Here, we monitored B. aeneus survival and corresponding αCOP expression in specimens. For survival analysis, starting n = 15 cages per treatment, each cage with eight insects. For both short-term and chronic dsRNA feeding, three cages (n = 3) were removed for qPCR analysis at both 3 and 6 days, and one insect was removed from each of the nine remaining cages at 12 days for each treatment.