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. 2021 Apr 6;7:38. doi: 10.1038/s41523-021-00245-5

Fig. 2. Inhibition of dUTPase enhances DNA damage induced by fluoropyrimidine and anthracycline chemotherapies.

Fig. 2

a MDA-MB-231 and b MDA-MB-468 cells were treated with 0.1 µM FUdR or 12.5 µM CV6-530 alone or in combination for 24 h when the media was replaced with drug-free media. At 4, 24, and 48 h, cells were fixed, stained, and imaged for ɣH2A.X (green) and 53BP1 (red). Line graphs represent the mean ± SEM percentage of cells positive for DNA damage (ɣH2A.X) or double-strand breaks (>5 co-localised ɣH2A.X/53BP1 foci) quantified from N = 3 independent experiments. >100 cells were scored per experiment. Representative immunofluorescent images of ɣH2A.X and 53BP1 marked DNA damage are shown on the right. c MDA-MB-231 cells were treated with 0.075 µM epirubicin for 4 h or 12.5 µM CV6-530 for 16 h alone or in combination. At 4, 8, 12 and 16 h, cells were fixed, stained and imaged for ɣH2A.X (green) and 53BP1 (red). Line graphs represent the mean ± SEM percentage cells positive for DNA damage (ɣH2A.X) or double-strand breaks (>5 co-localised ɣH2A.X/53BP1 foci) quantified from N = 3 independent experiments. >100 cells were scored per experiment Representative images of γ-H2AX and 53BP1 are shown on the right.