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. 2021 Apr 6;7:68. doi: 10.1038/s41420-021-00452-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Relative mRNA levels of CG6015 in the negative control (NC) and siCG6015 (siCG6015-741 and siCG6015-1331)-treated S2 cells to validate knockdown efficiency. b Immunostaining of NC and siCG6015-1331-treated S2 cells by using PH3 (red). c Percentages of PH3-positive cells in NC (n = 3) and siCG6015-1331-treated (n = 3) S2 cells. d CCK-8 test in NC and siCG6015-1331-treated S2 cells. e TUNEL (red) staining in NC and siCG6015-1331-treated S2 cells. f Percentages of TUNEL-positive cells in NC (n = 3) and siCG6015-1331-treated (n = 3) S2 cells. g Cell component analysis in NC and siCG6015-1331-treated S2 cells by flow cytometry. h Percentages of cell components in NC (n = 3) and siCG6015-1331-treated (n = 3) S2 cells. The Student’s t test was used for the statistical analysis. *P < 0.05; **P < 0.01; ***P < 0.001; n.s. not significant. Scale bar: 30 μm.