Fig. 7. Somatic Dsor1 inactivation causes ectopic dpERK expression in undifferentiated germ cells.

a–c Immunostaining of Vasa (red) and dpERK (green) in control (a) and tj > Dsor1 RNAi (b, c) testes. Images in (c) are enlarged views of the areas marked in (b). d Percentages of testes with germline dpERK signals in control (n = 20) and tj > Dsor1 RNAi (n = 15) testes. Chi-square test was used to evaluate for statistical differences of testes with germline dpERK signals. e The number of germline dpERK signals in control (n = 10) and tj > Dsor1 RNAi (n = 10) testes. The Student’s t test was used for statistical differences of the number of germline dpERK signals. ***P < 0.001. The W¹¹¹⁸ line was used as control. Scale bar: 20 μm.