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. 2021 Apr 6;12(4):351. doi: 10.1038/s41419-021-03595-x

Fig. 7. Inhibition of AURKA reverses cellular growth induced by SOCS2-AS1 loss.

Fig. 7

A CCK8 assay was performed in Ishikawa cells transfected with control or AURKA siRNA plasmid. B Colony-formation assays of Ishikawa cells transfected with control or AURKA siRNA plasmid. C Apoptotic rates of cells were analyzed in Ishikawa cells with control or AURKA siRNA plasmid. D Comet assays used to measure DNA damage in Ishikawa transfected with AURKA siRNA plasmid. E Immunoblotting of pH2AX in Ishikawa cells transfected with AURKA siRNA plasmid. F AURKA and SOCS2-AS1 double-knockdown rescue SOCS2-AS1 loss-mediated EC cell growth. CCK8 and colony-formation assays were measured in HEC-1A cells transfected with control, AURKA siRNA, SOCS2-AS1 siRNA, or co-transfected with both. G AURKA could reverse SOCS2-AS1-mediated EC cell growth inhibition. CCK8 and colony-formation assays were measured in Ishikawa cells transfected with control, AURKA, SOCS2-AS1, or co-transfected with both. H HEC-1A xenograft upon SOCS2-AS1 knockdown with or without knockdown of AURKA. I Growth of HEC-1A xenograft tumors was measured using tumor volume, and tumor size was monitored every 3 days. Data (n = 5) were analyzed using a paired-sample Student’s t test. J Tumor weight was measured in each group at the end of the experiment.