Fig. 3. Forced expression of SLC25A20 inhibited HCC cell invasion and migration through suppression of epithelial–mesenchymal transition (EMT).
A Cell migration ability was assessed using wound-healing assay in HLF and Huh-7 cells with treatment as indicated (SLC25A20, expression vector encoding SLC25A20; EV, empty vector). B Cell invasion ability was assessed by transwell matrigel-invasion assay in HLF and Huh-7 cells with treatment as indicated. C, D The expressions of EMT markers were determined by quantitative real-time PCR and Western blot analysis in HLF and Huh-7 cells with treatment as indicated.