Table 6.

GO Fig. 4A

		GO term name
Characteristics	Ontology	Transcript ID	Gene symbol	Gene description	FArg : GArg FC (log2)	FArg : FWt FC (log2)
Figure 4A, Category II, III 102 genes DIFFERENTIAL IN THE SPACEFLIGHT ADAPTATION IN ARG1 KO CELLS BUT SHOW NO DIFFERENTIAL EXPRESSION BETWEEN GENOTYPES WITHIN THE SPACEFLIGHT ENVIRONMENT	Cellular Component	cell periphery GO:0071944
		plasma membrane GO:0005886
		cell-cell junction GO:0005911
		AT1G32950		Subtilase family protein	3.3
		AT1G11690			3.3
		AT1G03920		Protein kinase family protein	2.9
		AT3G07320		O-Glycosyl hydrolases family 17 protein	2.8
		AT4G12770		Chaperone DnaJ-domain superfamily protein	2.4
		AT2G38330		MATE efflux family protein	1.7
		AT5G24970		Protein kinase superfamily protein	1.6
		AT1G78820		D-mannose binding lectin protein	1.2
		AT3G20410	CPK9	calmodulin-domain protein kinase 9	1.0
		AT3G13520	ATAGP12	arabinogalactan protein 12	−1.0
		AT1G60740		Thioredoxin superfamily protein	−1.0
		AT5G65390	AGP7	arabinogalactan protein 7	−1.2
		AT5G15350	ENODL17	early nodulin-like protein 17	−1.2
		AT1G12950	RSH2	root hair specific 2	−1.5
		AT5G14920		Gibberellin-regulated family protein	−1.6
		AT3G60270		Cupredoxin superfamily protein	−3.2
		endomembrane system GO:0012505
		Golgi apparatus GO:0005794
		AT1G74680		Exostosin family protein	2.4
		AT4G39890	RABH1c	RAB GTPase homolog H1C	1.4
		AT5G20280	SPSA1	sucrose phosphate synthase 1F	1.2
		AT1G49710	FUT12	fucosyltransferase 12	1.1
		AT4G08950	EXO	Phosphate-responsive 1 family protein	−1.1
		AT5G12140	CYS1	cystatin-1	−1.3
		AT3G52940	HYD2	Ergosterol biosynthesis ERG4/ERG24 family	−2.3
		membrane-bounded organelle GO:0043227
		intracellular membrane-bounded organelle GO:0043231
		AT2G40260		Homeodomain-like superfamily protein	3.3
		AT1G16500		unknown	2.8
		AT5G63700		zinc ion binding;DNA binding	2.8
		AT1G54230		Winged helix-turn-helix transcription repressor	2.8
		AT3G51470		Protein phosphatase 2C family protein	2.8
		AT1G18485		Pentatricopeptide repeat (PPR)	2.6
		AT1G03060	SPI	Beige/BEACH domain;WD domain, G-beta repeat	2.4
		AT3G62110		Pectin lyase-like superfamily protein	2.4
		AT4G17150		alpha/beta-Hydrolases superfamily protein	2.2
		AT5G14600		S-adenosyl-LMet-dependent methyltransferase	2.1
		AT2G43010	SRL2	phytochrome interacting factor 4	2.0
		AT1G70480		Domain of unknown function (DUF220)	1.7
		AT5G02440		unknown	1.3
		AT5G22880	HTB2	histone B2	1.3
		AT2G38390		Peroxidase superfamily protein	1.3
		AT2G38800		Plant calmodulin-binding protein-related	1.2
		AT5G43150			1.1
		AT3G53710	AGD6	ARF-GAP domain 6	1.1
		AT5G38850		Disease resistance protein (TIR-NBS-LRR class)	1.1
		AT4G33980			1.1
		AT2G43500		Plant regulator RWP-RK family protein	1.1
		AT4G36620	HANL2	GATA transcription factor 19	−1.1
		AT3G60530	GATA4	GATA transcription factor 4	−1.1
		AT5G15910		NAD(P)-binding Rossmann-fold	−1.3
		ORF111B			−1.3
		AT1G69690	TCP15	TCP family transcription factor	−1.4
		AT1G69010	BIM2	BES1-interacting Myc-like protein 2	−1.9
		AT3G43280			−1.9
		AT4G35070		SBP (S-ribonuclease binding protein)	−2.1
		AT3G09060		Pentatricopeptide repeat (PPR)	−2.3
		AT2G19460		Protein of unknown function (DUF3511)	−2.4
		AT5G38350		Disease resistance protein (NBS-LRR class)	−2.5
		ORF121A			−2.6
		AT5G53840		F-box/RNI-like/FBD-like domains	−2.9
		AT1G71050	HIPP20	Heavy metal transport/detoxification	−3.5
		plastid GO:0009536
		chloroplast GO:0009507
		AT2G24880		Plant self-incompatibility protein S1 family	2.8
		AT1G29395	COR414-TM1	COLD REGULATED 314 INNER MEMBRANE 1	2.4
		AT2G36830	TIP1;1	gamma tonoplast intrinsic protein	1.6
		AT1G10070	BCAT-2	branched-chain amino acid transaminase 2	1.4
		AT3G11560		LETM1-like protein	1.2
		AT1G66090		Disease resistance protein (TIR-NBS class)	−1.0
		AT4G05390	RFNR1	root FNR 1	−1.2
		AT1G60950	FED A	2Fe-2S ferredoxin-like superfamily protein	−1.5
		AT4G24090			−2.6
		AT5G59410		Rab5-interacting family protein	−2.9
		AT2G31270	CDT1A	homolog of yeast CDT1 A	−3.2
		AT4G17600	LIL3:1	Chlorophyll A-B binding family protein	−3.6
	Biological Process	localization GO:0051179
		single-organism localization GO:1902578
		establishment of localization GO:0051234
		transport GO:0006810
		single-organism transport GO:0044765
		AT1G80510		Transmembrane amino acid transporter	2.8
		AT2G38330		MATE efflux family protein	1.7
		AT2G36830	TIP1;1	gamma tonoplast intrinsic protein	1.6
		AT1G63440	HMA5	heavy metal ATPase 5	1.4
		AT5G20280	SPSA1	sucrose phosphate synthase 1F	1.2
		AT3G05030	NHX2	sodium hydrogen exchanger 2	1.2
		AT3G10520	NSHB2	haemoglobin 2	−1.4
		AT1G12950	RSH2	root hair specific 2	−1.5
		AT1G71050	HIPP20	Heavy metal transport/detoxification	−3.5
		signaling GO:0023052
		cell communication GO:0007154
		AT1G03060	SPI	Beige/BEACH domain; WD domain, G-beta repeat protein	2.4
		AT4G37580	UNS2	Acyl-CoA N-acyltransferases (NAT)	2.3
		AT2G43010	SRL2	phytochrome interacting factor 4	2.0
		AT5G38850		Disease resistance protein (TIR-NBS-LRR class)	1.1
		AT3G20410	CPK9	calmodulin-domain protein kinase 9	1.0
		AT3G16570	RALF23	rapid alkalinization factor 23	−1.2
		AT5G14920		Gibberellin-regulated family protein	−1.6

The significant GO terms assigned with AgriGO and gProfiler to 102 Corrected genes, which showed the expression change in the physiological adaptation to the spaceflight environment in ARG1 KO cells but no differential expression in spaceflight transcriptome between the ARG1 KO and WT cells. Blank cells indicate that the gene was not significantly differentially expressed in a respective comparison group. Gene duplicates within oncology were removed and assigned to the most specific available GO term class.