TABLE 2.
Definition of the spectral assignment | Classification |
Peak variation (cm–1) |
||
Control | 1 × MIC | 2 × MIC | ||
C-O, C-C str, C-O-H, C-O-C def of carbohydrates | Glycogen and nucleic acids | 1064.24 | 1068.79 | 1081.48 |
P = O str (asym) of > PO2 phosphodiesters | Mainly nucleic acids | 1234.36 | 1236.82 | 1237.94 |
C = O str (sym) of COO | amino acid side chains, fatty acids | 1388.36 | 1389.18 | 1390.14 |
Amide II (protein N–H bend, C–N stretch) | α helices | 1528.37 | 1533.94 | 1543.78 |
Amide I of β-pleated sheet structures | β-pleated sheet | 1636.55 | 1647.42 | 1648.15 |
C-H str (asym) of > CH2 | Mainly lipids | 2929.72 | 2933.88 | 2935.29 |
O-H str of hydroxyl groups | Polysaccharides, proteins | 3278.44 | 3283.42 | 3287.68 |
Str, stretching; def, deformation; sym, symmetric; asym, antisymmetric.