Figure 1.
Experimental design. hBM-MSCs were isolated from human bone marrow and fractionated according to CD146 presence. CD146− cells were induced into an osteogenic phenotype (OLCs) and CD146+ cells were determined to be pericyte-like cells (PLCs). Transwell migration assays were performed to determine if 3384T were attracted to OLCs. Additionally, proliferation assays were performed where 3384T breast cancer cells were cultured for five days in OLCCM and PLCCM. Finally, transwell co-cultures of each OLCTW and PLCTW were performed with 3384T breast cancer cells as well as cultures of 3384T breast cancer cells in OLCCM and PLCCM. The 3384T cells were collected on day 1 and day 3 for gene expression interrogation. (A color version of this figure is available in the online journal.)