Figure 2.

Main UGGT motions and conformational coordinates

(A) Simplified representation of CtUGGT overall movements. “Clamping” movement between domains TRXL3 and TRXL1; “bending” movement between TRXL2 and the core comprising domains GT24-βS1-βS2-TRXL4; “twisting” movement of TRXL2 with respect to TRXL3. The gray area represents the strong structural inter-domain orientation invariance of the TRXL4-βS1-βS2-GT24 domains.

(B–D) Conformational coordinates (CCs) for describing CtUGGT conformational states: TRXL1 (magenta), TRXL2 (blue), and TRXL3 (cyan). (B) Along “CC1,” the “clamping” coordinate measures the openness of the cleft between the TRXL1 and TRXL3 domains. (C) Along “CC2,” the “bending” coordinate measures the distance between the TRXL2 and GT24 domains across the central saddle. (D) Along “CC3,” the “twisting” coordinate changes with the relative orientation of the TRXL2 and TRXL3 domains.

(B), (C), and (D) made in VMD (Cross et al., 2009).