Fig. 8.

Immunohistological images and semi-quantitative analyses of specific markers of in vitro tissue-like constructs after 21 days of culture. (A)–(C) Nestin and tublin staining in NE-4C constructs and semi-quantitative analyses of their immunoreactivity (n = 5, error bars, mean ± SD). (D)–(F) Desmin and fast myosin skeletal heavy chain staining in C2C12 constructs and semi-quantitative analyses of their immunoreactivity (n = 5, error bars, mean ± SD). (G)–(I) Collagen II and alcian blue staining in chondrocyte constructs and their semi-quantitative analyses (n = 5, error bars, mean ± SD). Yellow and orange squares: the position of the high-magnification images below. Dotted lines, microfilament range. *P < 0.05, **P < 0.01, ***P < 0.001, between different constructs. Images of negative controls were shown in Fig. S11. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)