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. 2021 Mar 24;9:653869. doi: 10.3389/fchem.2021.653869

TABLE 1.

Comparison of the aptasensor with previously reported methods for detection of AFM1.

Detection methods Transduction principle LOD References
Instrumental methods HPLC 6 × 10−3 ng/mL Wang et al. (2012)
SPE-UPLC–MS/MS 0.3 × 10−3 ng/mL Wang and Li (2015)
Antibody-based methods Impedimetric biosensor 1 ng/mL Dinakaya et al. (2011)
Enzyme immunoassay 5.0 × 10−3 ng/mL Anfossi et al. (2008)
Electrochemical immunosensors 1.0 × 10−6 ng/mL Neagu et al. (2009)
Aptamer-based methods Immunochromatographic assay 0.05 ng/mL Wu et al. (2016)
Label free polyaniline based aptasensor 1.98 × 10−3 ng/mL Nguyen et al. (2013)
Electrochemical impedance spectroscopy aptasensor 1.15 × 10−3 ng/mL Istamboulia et al. (2016)
RT-qPCR based aptasensor 0.03 × 10−3 ng/mL Guo et al. (2016)
Structure switching signaling aptamer assay 5.0 × 10−3 ng/mL Sharma et al. (2016)
Visual electrochemiluminescence biosensing 0.05 ng/mL Khoshfetrat et al. (2017)
Aptamer-based fluorescence-quenching assay 0.5 ng/mL This work