Figure 1.

Schematic workflow of infected and non-infected HbAA and HbAS erythrocytes proteome and phosphoproteome experimental procedures – HbAA and HbAS erythrocytes were collected and infected in vitro with the 3D7 P. falciparum strain. Mature trophozoite/schizont-infected RBCs were collected by MACS (45% parasitemia) and lysed to produce ghosts and collect parasites. Membrane erythrocyte lysates were analyzed by western blot. For mass spectrometry, after trypsin digestion of proteins, phosphopeptides were enriched on TiO₂ columns and analyzed by LC-MS/MS. Total proteomes of erythrocyte and parasite proteins were also generated. i, infected; ni, non-infected.