Skip to main content
. 2021 Mar 24;11:603533. doi: 10.3389/fonc.2021.603533

Figure 2.

Figure 2

Conditioned medium (CM) of GC-MSCs treated with DIM (50 μM for 48 h) promoted gastric cancer progression. CM of GC-MSCs pre-treated with 50 μM (50 μM-CM) or the same volume of DMSO (0 μM-CM) for 48 h is collected. (A) SGC-7901, MGC80-3, and HGC-27 cells are co-cultured with CM of GC-MSCs for 48 h, and then collected for colony formation assay for 7 days, Original magnification, 40 X. (B) The migratory ability of SGC-7901, MGC80-3, and HGC-27 cells treated with the CM of GC-MSCs is evaluated using trans-well migration assay. Original magnification, 100 X. (C, D) MGC80-3 are pretreated with CM of GC-MSCs for 48 h respectively, with PBS used as a control and then treated with 50 μM DIM for 48 h. Cells are collected and labeled with Annexin V/PI and detected using flow cytometry. (E) MGC80-3 are pre-treated with CM of GC-MSCs for 48 h and treated with 50 μM DIM, then the growth curve is compared with these in MSC80-3 treated 50 μM DIM or the same volume of PBS, or cultured with the L-DMEM. (F) MGC80-3 pretreated with CM of GC-MSCs, or the same volume of PBS. Real-time RT-PCR detects the expression of drug resistance genes MDR, MRP, and LRP. (***P < 0.001 compared with the control group).