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. 2021 Mar 24;11:603533. doi: 10.3389/fonc.2021.603533

Figure 4.

Figure 4

DIM increases the expression of NFκB in GC-MSCs by inhibiting β-TrCP. (A) Schematic diagram of the βTRCP-NFκB signaling pathway. (B) Western blot analysis of the expression of β-TrCP in GC-MSCs pretreated with 1, 10, 25, 50, and 100 μM DIM or the same volume of DMSO (0 μM) for 48 h. (C) The level of β-TrCP in GC-MSCs treated with 50 μM DIM or the same volume of DMSO (0 μM) for 15 min, 30 min, 1 h, 12 h, 24 h, or 48 h are determined using Western blot. (D) Real-time RT-PCR is used to detect the β-TrCP gene in GC-MSCs pretreated with 1, 10, 25, and 50 μM DIM or the same volume of DMSO (0 μM) for 48 h. (E) The expression of IκBα, β-TrCP, and p-NF-κB in GC-MSCs pretreated with 50 μM DIM or the same volume of DMSO (0 μM) for 48 h are detected using western blotting (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group).