Table 1.
Gen targetet | ID transcript Ensembl Mesocricetus auratusa | Primer forward | Primer reverse | Size of products | Eb |
---|---|---|---|---|---|
Cb1r | ENSMAUG00000014040 | TGTTGACTTCCATGTGTTCCA | GGTCTGGTGACGATCCTCTT | 171 | 1.15 |
Actb | ENSMAUG00000008763 | AGCCATGTACGTAGCCATCC | ACCCTCATAGATGGGCACAG | 105 | 2.03 |
List of primers used for RT-qPCRs, indicating the location of each primer in the corresponding Ensembl sequences of the Syrian hamster (a). qPCR primer efficiency (Eb) was calculated according to the following equation: E = 10(−1/slope).