FIGURE 1.

SYQP stimulated human TLR4 in HEK-BLUE hTLR4 cells. (A) SYQP could activate NF-κB in HEK-BLUE hTLR4 but not HEK-BLUE NULL cells. (B) SYQP showed no cytotoxicity. Cells were treated with SYQP for 24 h, and cell viability was evaluated by MTS assay. (C) SYQP pretreatment could not inhibit LPS-induced NF-κB activation in HEK-BLUE hTLR4 cells. Cell culture supernatants were mixed with QUANTI-BLUE substrate to test the level of SEAP, which represented the activity of NF-κB in the HEK-BLUE reporter cells. All of the data were normalized to the 1 μg/ml LPS-treated group, which represented the maximum response of the cells. All of the results are presented as the mean ± SD (n = 3).