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. 2021 Mar 24;12:609059. doi: 10.3389/fphar.2021.609059

FIGURE 2.

FIGURE 2

SYQP stimulated human TLR4 in PMA-induced THP-1 cells. (A) Effects of SYQP on viability of THP-1 cells. Cells were treated with SYQP for 24 h, and cell viability was evaluated by MTS assay. (B) SYQP increased the secretion of TNF-α from THP-1 cells. The cells were treated with SYQP at the indicated concentration for 24 h. Cell culture medium was collected, and the secretion levels of cytokines were detected by ELISA. ***p < 0.001 vs. control (the concentration of SYQP was zero). (C) TLR4 antagonist TAK-242 decreased the secretion of TNF-α promoted by SYQP. The cells were treated with 100 μg/ml SYQP alone or combined with TAK-242 for 24 h. LPS at 1 μg/ml was used as a positive control. Cell culture medium was collected, and the secretion levels of TNF-α were detected by ELISA. ***p < 0.001 vs. cells treated without TAK-242. All of the results are presented as the mean ± SD (n = 3). (D) SYQP induced mRNA expression of both MyD88-dependent and TRIF-dependent cytokines in THP-1 cells. The cells were treated with 1, 10, 100 μg/ml SYQP or 1 μg/ml LPS for 6 h. After that, mRNA was isolated, and the gene expression levels of cytokines were determined by real-time PCR. The results are presented as the mean ± SD (n = 3). **p < 0.01, ***p < 0.001 vs. control.