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. 2021 Mar 8;24(4):102283. doi: 10.1016/j.isci.2021.102283

Figure 2.

Figure 2

VLGR1 is localized at FAs

(A and B) Double labeling immunofluorescence of VLGR1 (green) and vinculin (magenta) in hTERT-RPE1 cells revealed a concentration of VLGR1 at the tip of F-actin stained by TRITC-phalloidin (red) (A) and in different forms of FAs (B). Pearson correlation coefficient R calculated in magnified images for white dotted region quantifies the degree of co-localization.

(C and D) Normalized fluorescence intensity plots of VLGR1 and F-actin (C) and VLGR1 and vinculin (D) share common peaks along the depicted blue line (ROI) in the magnified images indicating co-localization of both proteins.

(E) In a proximity ligation assay (PLA), the VLGR1-vinculin interaction dots (green) are in close proximity to F-actin. The boxed areas in the overlays are shown magnified. Nuclei are stained with DAPI (blue); scale bars: 25 μm, 5 μm magnified images. See also Figures S1–S4.