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The high-altitude deer mouse Hif-2α T755M mutation impairs its interaction with Cbp.A–C and E, HEK293FT or (D) N2a cells were transfected and then exposed (A and B) to 0.5% O2 for 18 h, or (C–E) normoxia. A–C and E, flag-tagged or (D) hemagglutinin (HA)-tagged proteins were immunoprecipitated and Western blotting performed. CBP, CREB-binding protein; Hif-2α, hypoxia-inducible factor-2α.
