Figure 11.

Ligation efficiency of the repair intermediates with 3'-preinserted dA:G and dG:A mismatches by low-fidelity LIG1.A and C, lane 1 is the negative enzyme control of the nicked DNA substrate with 3'-dA:G (A) and 3'-dG:A (C) mismatches. Lanes 2 to 5, 6 to 9, 10 to 13, and 14 to 17 show the reaction products by LIG1 WT, E346A, E592A, and E346A/E592A (EE/AA) mutants, respectively, for 3'-dA:G (A) and 3'-dG:A (C) substrates, obtained at the time points 0.5, 3, 5, and 10 min. B and D, the graphs show the time-dependent changes in the ligation products, and the data are presented as the averages from three independent experiments ± SDs. LIG1, DNA ligase I.