Figure 13.

Removal of 5'-AMP by APTX from the ligation failure products with 3'-8-oxodG.A, lane 1 is a size marker that corresponds to the oligonucleotide without an AMP moiety. Lanes 2 and 9 are the negative enzyme controls of the nicked DNA substrates with 5'-AMP and 3'-8-oxodG opposite template base A or C, respectively. Lanes 3 to 8 and 10 to 15 show the products of 5'-AMP removal from 3'-8-oxodG:A and 3'-8-oxodG:C substrates, respectively, obtained at the time points 0.5, 1, 3, 5, 8, and 10 min. B, the graph shows the time-dependent changes in the products of 5'-AMP removal, and the data are presented as the averages from three independent experiments ± SDs. C, illustrations of the nicked DNA substrate with 5'-AMP and 3'-8-oxodG and the products observed in the APTX reaction. APTX, aprataxin.