Fig. 3.

PELP1 is essential for TFAP2C‐mediated oncogenic functions and therapy resistance. (A) Total lysates from MCF7 or ZR75 cells expressing TFAP2C or PELP1 shRNA were analyzed by western blotting for the status of TFAP2C, ERα, and PELP1. Data are shown from two independent experiments. (B) Equal number of MCF7 or ZR75 model cells expressing TFAP2C or/and PELP1 shRNA was plated in six‐well plates and cultured for 14 days, and the number of colonies for each group was counted (n = 3). (C) Anchorage‐independent growth potential of the MCF7 cells expressing TFAP2C or/and PELP1 shRNA was measured by soft agar colony formation assay. Representative photographs and quantitation of the soft agar colony formation are shown, scale bar = 100 µm. (D) MCF7 or ZR75 model cells expressing TFAP2C or/and PELP1 shRNA were treated with E2 + Fulvestrant (ICI) for 5 days and then cultured for seven subsequent days. The number of colonies for each group was counted. Data are shown as the means ± SEM of three experiments. *P < 0.05; ***P < 0.001; ****P < 0.0001 by Student's t‐test in B–D.