Fig. 4.

PELP1 is needed to facilitate optimal histone methyl modifications at the RET promoter by TFAP2C. (A) Schematic representation of TFAP2C‐binding sites in the RET promoter based on published TFAP2C ChIP‐seq data in the RET locus of MCF7 cells. (B) Primary ChIP was conducted using TFAP2C antibody followed by Re‐ChIP using PELP1 antibody. The status of TFAP2C and PELP1 at the RET promoter was determined by qPCR using primers spanning four TFAP2C‐binding sites. (C) Status of the TFAP2C, PELP1, and histone methyl marks H3K4me3 and H3K9me3 in theRET promoter was determined by qPCR using primers spanning the four TFAP2C‐binding sites. Isotype‐matched IgG ChIP was used as control. Data are shown as the means ± SEM of two experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by Student's t‐test in B–C.