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. 2021 Jan 26;15(4):1130–1145. doi: 10.1002/1878-0261.12882

Fig. 3.

Fig. 3

Wee1 amplification in GLC4‐resistant cells is highly correlated with the degree of resistance. Cell viability of H792LYR (A) or GLC4LYR cells (B) by CellTiter‐Glo. Cells were cultured without prexasertib for different time periods as indicated. Data are presented as mean ± SD of three independent experiments. (C) GLC4LYR cells were cultured without prexasertib for different time periods, Wee1 protein expression was detected by WB. (D) Cell cytotoxicity of prexasertib for 19 single clones of GLC4LYR by CellTiter‐Glo. (E) Wee1 mRNA expression of 10 GLC4LYR single clones, detected by qRT‐PCR. (F) Wee1 DNA copy number of 10 GLC4LYR single clones, analyzed by DNA copy number assay. (G) 18 GLC4LYR single clones in which Wee1 protein expression was detected by WB. (H) Graphical representation of the western blot (G) is shown in the bar graph where the band intensity of Wee1 is normalized to the α‐tubulin.