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. 2021 Mar 9;15(4):866–886. doi: 10.1002/1878-0261.12910

Fig. 7.

Fig. 7

In vitro experiments on the effects of and thymocyte selection‐associated high‐mobility group box (TOX) on the antitumor function of human T cells. (A) Schematic of linear regression revealed a positive correlation between TOX expression level and (programmed death protein 1) PDCD1 expression level in The Cancer Genome Atlas (TCGA) expression profiles (t‐test, P < 0.01. n = 566). (B) The overexpression of TOX led to reduced expression of TCF7, KLF2, LEF1, and CCR7, and increased expression of PDCD1, TIGIT, CTLA4, HAVCR2, TNFRSF9, and ID2. (C) Line graph of apoptosis assays of tumor cells cocultured for 12 h using fluorescence‐activated cell sorting (t‐test, P < 0.01, n = 3) . Data are presented as means ± SD. (D) Proliferation efficiency of three types of CD8+ T cells: control (Ctrl), overexpressing (OE) CD8+ T cells, PDCD1 OE CD8+ T cells, and TOX‐overexpressing OE CD8+ T cells detected using carboxyfluorescein succinimidyl amino ester (CFSE).