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. 2021 Feb 26;15(4):1162–1179. doi: 10.1002/1878-0261.12906

Fig. 3.

Fig. 3

DDX21 induces neuroblastoma cell proliferation through upregulating CEP55 expression. (A–B) DOX‐inducible control shRNA, DDX21 shRNA‐1, or DDX21 shRNA‐2 BE(2)‐C and Kelly cells were treated with control or DOX for 72 h, followed by Alamar blue assays (A) or BrdU incorporation assays (B). (C‐E) BE(2)‐C and Kelly cells were transfected with control siRNA, CEP55 siRNA‐1, or CEP55 siRNA‐2 for 48 h (C) or 72 h (D, E), followed by RT‐PCR (C), Alamar blue assays (D), or BrdU incorporation assays (E). (F–G) DOX‐inducible control shRNA, DDX21 shRNA‐1, or DDX21 shRNA‐2 BE(2)‐C and Kelly cells were treated with vehicle control or DOX for 14 days. Cell colonies were fixed with methanol and stained with crystal violet (F), and the numbers of colonies were counted (G). Error bars represent standard error. * indicates P < 0.05, **P < 0.01, and ***P < 0.001.