Fig. 4.

DDX21 and CEP55 play a role in neuroblastoma cell cytoskeletal stability. (A–B) DOX‐inducible control shRNA, DDX21 shRNA‐1, or DDX21 shRNA‐2 BE(2)‐C and Kelly cells were treated with vehicle control or DOX for 72 h, followed by immunostaining with an anti‐α‐tubulin antibody and counter‐staining of cell nuclei with DAPI (A). The areas covered by α‐tubulin filaments, relative to nucleus, were quantified (B). (C–D) BE(2)‐C and Kelly cells were transfected with control siRNA, CEP55 siRNA‐1, or CEP55 siRNA‐2 for 72 h followed by immunostaining with an anti‐α‐tubulin antibody and counter‐staining of cell nuclei with DAPI (C). The areas covered by α‐tubulin filaments, relative to nucleus, were quantified (D). Error bars represent standard error. * indicates P < 0.05, ** P < 0.01, and *** P < 0.001. Scale bar: 10 µm.