Fig. 4.

miR‐378 activated AKT signaling via targeting Smad4. (A) The predicted binding sites between miR‐378 and Smad4 mRNA. (B) Relative luciferase activity of WT‐Smad4 and MUT‐Smad4 in cells transfected with mimics NC, miR‐485‐3p mimics. (C) qRT‐PCR analysis to measure Smad4 mRNA levels in SKOV3 and CAOV3 cells transfected with circATRNL1 overexpressing vector. Relative mRNA level was normalized to GAPDH. (D) qRT‐PCR analysis to measure Smad4 mRNA levels in SKOV3 and CAOV3 cells transfected with miR‐378 mimics. Relative mRNA level was normalized to GAPDH. (E) Western blot analysis of protein levels of Smad4, p‐AKT, and AKT. The protein levels were normalized to GAPDH. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *P < 0.05, **P < 0.01, and ***P < 0.001.