Fig. 7.

Overexpression of circATRNL1 inhibited ovarian cancer abdominal metastasis via miR‐378/Smad4 in vivo. (A) Representative images of tumor in mice‐bearing SKOV3 and CAOV3 cells transfected with vector and circATRNL1. n = 5. (B) Quantifications of the number of tumor nodules in the abdomen. (C) Quantification of tumor weight. (D) Western blot analysis of protein levels of Smad4, p‐AKT, AKT, c‐Myc, Cyclin D1, MMP‐2, and MMP‐9 in tumor tissues from different groups of mice. The protein levels were normalized to GAPDH. (E) Immunohistochemistry analysis of Smad4 and CD31 levels in tumor tissues from different groups of mice Scale bar: 50 μm. The results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *P < 0.05, **P < 0.01, and ***P < 0.001.