Fig. 2.

TMEM92‐AS1 regulates GC cell proliferation and migration in vitro. (A) Interference efficiency of si‐TMEM92‐AS1 1#, 2# and 3#. (B) MTT assays were performed to detect cell proliferation of BGC823 and SGC7901 after transfection with si‐TMEM92‐AS1 2# and 3#. (C) Colony formation assays of BGC823 and SGC7901 transfected with si‐TMEM92‐AS1 2# and 3# (scale bar: 5 mm). (D) Edu assays of BGC823 and SGC7901 transfected with si‐TMEM92‐AS1 2# and 3# (scale bar: 100 μm). (E,F) 48 h after transfection, cell cycle and apoptosis cell percentage were analysed by flow cytometry. (G,H) Transwell assays were used to detect changes of migration and invasion abilities in BGC823 and SGC7901 cells (scale bar: 100 μm). Student’s t‐test was used to compare differences between different groups: *P < 0.05, **P < 0.005. For all of these experiments, values were mean ± SD (error bars) of triplicate samples in representative experiments.