Fig. 3.

TMEM92‐AS1 regulates expression of cellular functional genes. (A B) RT‐qPCR and western blot assays were used to detect expression changes of cellular functional genes and proteins. (C) ShCtrl and shTMEM92‐AS1 were stably transfected into BGC823 cells and then injected into subcutaneous of nude mice. Tumour volumes were measured every 2 days after macroscopic formation of subcutaneous tumours (scale bar: 100 μm). (D) Nuclear and cytosolic separation assays were used to detect distribution of TMEM92‐AS1 in BGC823 and SGC7901 cells, GAPDH was used as a cytoplasmic marker and U1 was used as a nuclear marker. (E) Transcriptome sequencing results of gene transcripts altered (> 1.0‐fold) after knockdown of TMEM92‐AS1 by si‐TMEM92‐AS1 2# in BGC823 cells. (F) Number of genetic changes found by transcriptome sequencing. (G) Gene ontology analysis of all the genes altered after TMEM92‐AS1 knockdown. *P < 0.05, **P < 0.005 by Student’s t‐test. For all of these experiments, values were mean ± SD (error bars) of triplicate samples in representative experiments.