Fig. 4.

CCL5 was confirmed as a downstream target gene of TMEM92‐AS1. (A,B) RT‐qPCR and western blot were used to verify changed genes and proteins after TMEM92‐AS1 knockdown. (C) MTT assays were performed to detect the effect of knockdown of CCL5 and overexpression of TMEM92‐AS1 on cell proliferation. (D) Colony formation assays of knockdown of CCL5 and overexpression of TMEM92‐AS1 in BGC823 and SGC7901 cells (scale bar: 5 mm). (E) Edu assays of knockdown of CCL5 and overexpression of TMEM92‐AS1 in BGC823 and SGC7901 cells (scale bar: 100 μm). (F) RT‐qPCR and western blot were used to detect changes in TMEM92‐AS1‐related functional genes expression after knockdown of CCL5. *P < 0.05, **P < 0.005 by Student’s t‐test. For all of these experiments, values were mean ± SD (error bars) of triplicate samples in representative experiments.