Fig. 5.

YBX1 acted as a RBP for TMEM92‐AS1. (A) RIP experiments were performed for YBX1/KDM4A/hnRNPK/VDR/BMI1/LSD and DNMT1 and the coprecipitated RNA was performed by qRT‐PCR for TMEM92‐AS1. (B) RT‐qPCR was used to detect YBX1 mRNA level after TMEM92‐AS1 knockdown. (C) RT‐qPCR and western blot were performed to detect CCL5 expressions after YBX1 knockdown. (D) MTT and colony formation assays were performed to verify the effect of YBX1 on cell proliferation. (E) RT‐qPCR and western blot were performed to detect the effect of TMEM92‐AS1 overexpression and YBX1 knockdown on CCL5 expression. (F,G) ChIP results of YBX1 of the promoter region of CCL5 after si‐TMEM92‐AS1 2# in BGC823 cell. (H) The expression of TMEM92‐AS1, YBX1 and CCL5 was detected in 30 GC tissues and the expressions of these three were positively correlated. *P < 0.05, **P < 0.005 by Student’s t‐test. For all of these experiments, values were mean ± SD (error bars) of triplicate samples in representative experiments.