10 M NaOH |
20g NaOH, fill up to 50 mL with double distilled H2O (ddH2O) |
2 M Tris-HCl, pH 7.5 |
242.2 g Tris base, ∼120 mL conc. HCl to pH 7.5, fill up to 1 L with ddH2O |
0.5 M Tris-HCl, pH 6.8 |
30.3 g Tris base, ∼40 mL 6 N HCl to pH 6.8, fill up to 500 mL with ddH2O) |
0.5 M PIPES, pH 6.8 |
15.1 g PIPES, 7–8 mL 10 N NaOH to pH 6.8, fill up to 100 mL with ddH2O |
0.5 M EDTA, pH 8.0 |
93.06 g EDTA·2Na·2H2O, 10 g NaOH to pH 8.0, fill up to 500 mL with ddH2O |
50 mM EGTA, pH 7.6 |
1.90 g EGTA, adjust pH with 10 M NaOH, fill up to 100 mL with ddH2O |
5 M NaCl |
292.2 g NaCl, fill up to 1 L with ddH2O |
1 M MgCl2
|
20.3 g MgCl2·6H2O, fill up to 100 mL with ddH2O |
1 M CaCl2
|
14.7 g CaCl2·2H2O, fill up to 100 mL with ddH2O |
1 M NaHCO3
|
8.40 g NaHCO3, fill up to 100 mL with ddH2O |
0.2 M Na2HPO4
|
71.63 g Na2HPO4·12H2O, fill up to 1000 mL with ddH2O |
0.2 M NaH2PO4
|
15.6 g NaH2PO4·2H2O, fill up to 500 mL with ddH2O |
0.2 M sodium phosphate buffer |
700 mL 0.2 M Na2HPO4, to pH 7.0 with 350–450 mL 0.2 M NaH2PO4
|
1 M sodium butyrate |
5.5 g sodium butyrate, fill up to 50 mL with ddH2O |
10% NP-40 |
10 mL NP-40 (IGEPAL CA630), 90 mL ddH2O, warm to 40°C∼50°C to dissolve NP-40 |
10% Triton X-100 |
10 mL Triton X-100, 90 mL ddH2O, warm to 40°C∼50°C to dissolve Triton X-100 |
10% SDS |
10 g SDS, fill up to 100 mL with ddH2O |
1 M DTT |
1.542 g DTT, fill up to 10 mL with ddH2O, store in aliquots at −20°C for up to 1 year |
1% bromophenol blue (BPB) |
1 g BPB, fill up to 100 mL with ddH2O |
1% xylene cyanol FF |
1 g xylene cyanol FF, fill up to 100 mL with ddH2O |
1U/μL MNase |
reconstitute lyophilized MNase with an MNase storage buffer, store at −20°C |