Protocol for fractionation-assisted native ChIP (fanChIP) to capture protein-protein/DNA interactions on chromatin

. 2021 Mar 23;2(2):100404. doi: 10.1016/j.xpro.2021.100404

2 × sample buffer

Reagent	Final concentration	Stock concentration	Amount in 100 mL
Tris-HCl pH 6.8	125 mM	0.5 M	25 mL
SDS	4%	10%	40 mL
2-mercaptoethanol (2-ME)			10 mL
Bromophenol blue	0.02%	1%	2 mL
ddH₂O			23 mL

Store at 23°C–25°C for up to 6 months.