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Lysis buffer, -DTT
| Reagent | Final concentration | Stock concentration | Amount in 1 L |
|---|---|---|---|
| Sodium phosphate pH 7.0 | 20 mM | 0.2 M | 100 mL |
| Sodium pyrophosphate10·H2O | 30 mM | 13.38 g | |
| NaCl | 250 mM | 5 M | 50 mL |
| NaF | 10 mM | 0.42 g | |
| EDTA pH 8.0 | 5 mM | 0.5 M | 10 mL |
| Glycerol | 10% | 100 mL | |
| NP-40 | 0.1% | 10% | 10 mL |
| ddH2O | Up to 1 L |
Store at −20°C for up to 1 year.
