Reagent | Final concentration | Stock concentration | Amount in 10 mL |
---|---|---|---|
Tris-HCl pH 7.5 | 50 mM | 2 M | 250 μL |
DTT | 1 mM | 1 M | 10 μL |
NaCl | 50 mM | 5 M | 100 μL |
Glycerol | 50% | 5 mL | |
ddH2O | Up to 10 mL |
Store at −20°C for up to 1 year.
Reagent | Final concentration | Stock concentration | Amount in 10 mL |
---|---|---|---|
Tris-HCl pH 7.5 | 50 mM | 2 M | 250 μL |
DTT | 1 mM | 1 M | 10 μL |
NaCl | 50 mM | 5 M | 100 μL |
Glycerol | 50% | 5 mL | |
ddH2O | Up to 10 mL |
Store at −20°C for up to 1 year.