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MNase buffer, complete
| Reagent | Final concentration | Stock concentration | Amount in 50 mL |
|---|---|---|---|
| 2 × MNase buffer | 1 × | 25 mL | |
| Sucrose | 0.3 M | 5.15 g | |
| DTT | 0.5 mM | 1 M | 25 μL |
| Sodium butyrate | 5 mM | 1 M | 250 μL |
| ddH2O | Up to 50 mL |
Add 2 tablets of cOmplete protease inhibitor cocktail EDTA, and store it at −20°C for up to 1 year.
