Lysis buffer, complete
| Reagent | Final concentration | Stock concentration | Amount in 50 mL |
|---|---|---|---|
| Lysis buffer | 50 mL | ||
| DTT | 0.5 mM | 1 M | 25 μL |
Add 1 tablet of cOmplete protease inhibitor cocktail EDTA, and store it at −20°C for up to 1 year.
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