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. 2021 Apr 15;526:111195. doi: 10.1016/j.mce.2021.111195

Fig. 2.

Fig. 2

Effect of LPS treatment in ATC7 cells co-cultured with THP1 cells on IL-6 mRNA expression in ATC7 cells. ATC7 cells were cultured alone (A) or co-cultured with THP1 cells (A and B) and treated with LPS for 24-h. Relative levels of IL-6 mRNA were measured in ATC7 cells by RTqPCR and GAPDH was used as a house-keeping gene. (A) Effect of increasing ATC7:THP1 cells ratio and LPS treatment (5 μg/mL) on IL-6 mRNA expression in ATC7 cells. Data are the mean ± SEM (n = 4/group) and are expressed as fold induction of untreated ATC7 (1: 0) cells. Data were analyzed by two-way ANOVA, followed by Tukey's multiple comparison test. (B) Effect of increasing doses of LPS in ATC7 cells co-cultured with THP1 cells (1:2 ratio) on IL-6 mRNA expression in ATC7 cells. Data are the mean ± SEM (n = 4/group) and are expressed as fold induction of untreated ATC7 cells co-cultured with THP1 cells (Ctrl). Data were analyzed by one-way ANOVA, followed by Tukey's multiple comparison test. Effect of LPS: *P < 0.05; **P < 0.01 vs Ctrl.