Skip to main content
. 2021 Feb 25;22(4):e51861. doi: 10.15252/embr.202051861

Figure 2. Expression of endogenous channels is patterned and contributes to depolarization anterior to the compartment boundary.

Figure 2

  • A–A″
    Expression of Rpk is increased anterior to the A‐P compartment boundary and in two rows of cells flanking the D‐V compartment boundary in the A compartment.
  • B–B″
    Blockade of DEG/ENaC channels by incubation in amiloride abolishes the stripe of increased DiBAC fluorescence along the A‐P compartment boundary (white arrowhead). White boxes indicate regions used to calculate the average DiBAC fluorescence intensity ratio across the compartment boundary = 0.98, standard deviation = 0.05, n = 5 discs. Increased fluorescence at the D‐V boundary in the A compartment is still observable (yellow arrowhead). The same vehicle was used as in experiments with ouabain, and the control is in Fig 1F.
  • C, C′
    Amiloride incubation does not diminish Rpk expression. White arrowhead indicates approximate position of the A‐P compartment boundary, yellow arrowhead indicates approximate position of the D‐V compartment boundary.
  • D, E
    Expression of rpk‐RNAi using dpp‐Gal4 results in diminished DiBAC fluorescence along the A‐P compartment boundary (white arrowhead), while increased fluorescence at the D‐V boundary in the A compartment is still observable (yellow arrowhead).

Data information: Scale bars are 100 µm, except in (B″), where scale bar is 50 µm.