Figure EV4. Manipulating levels of the ion channel Rpk impacts both Hh and Wg signal transduction.

• A, B
  Expression patterns of ap‐Gal4 (A) and rn‐Gal4 (B).
• C, D
  Immunostaining of Smo protein (red), full‐length Ci (light blue) (C–C″) and Ptc protein (D) in discs expressing ATPα^RNAi in the dorsal compartment. Brackets indicate tissue expressing RNAi and control tissue.
• E–F″
  Immunostaining of Smo protein (red) and full‐length Ci (light blue) in (E–E″) control discs and (F–F″) discs expressing rpk^RNAi for 48 h before dissection.
• G–H′
  Immunostaining of Ptc protein in (G, G′) control discs and (H, H′) discs expressing rpk^RNAi for 48 h before dissection.
• I–J″
  Immunostaining for Wg protein in (I–I″) control discs and (J–J″) discs expressing rpk^RNAi for 48 h before dissection.
• K–L′
  Immunostaining for Cut protein in (K, K′) control discs and (L, L′), discs expressing rpk^RNAi for 48 h before dissection.

Data information: All scale bars are 100 µm except (D), where scale bar is 50 µm.