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. 2021 Mar 3;22(4):e51400. doi: 10.15252/embr.202051400

Figure 4. Kinetochore–microtubule attachment stability is not required for initiating but is required for terminating spindle elongation.

Figure 4

  1. Live imaging of Zp3‐Cre Ndc80f/f oocytes injected with Ndc80‐WT, Ndc80‐9D or Ndc80‐9A. Z‐projection and 3D‐reconstructed images show microtubules (EGFP‐Map4, green) and chromosomes (H2B‐mCherry, magenta). Scale bar, 10 μm.
  2. Ndc80‐9D is defective for limiting spindle elongation. The aspect ratio (length/width) of 3D‐reconstructed spindles was measured over time (n = 18, 18, and 18 oocytes, respectively, from three independent experiments).
  3. Ndc80‐9D causes excessive spindle elongation. Spindle lengths measured after 3D reconstruction are shown (n = 18, 18, and 18 oocytes, respectively, from three independent experiments). Two‐tailed Student’s t‐test was performed. **P < 0.01, ****P < 0.0001.

Data information: Means ± SD are shown. See also Movie EV8.

Source data are available online for this figure.