Figure 5. Validation of glutamatergic FXR2P mRNA targets.

1. Representative Western blot and quantification of GluK5, PSD‐95, GluN2B, and mGluR5 proteins. Endogenous GluK5, PSD‐95, GluN2B, and mGluR5 in Fxr2 KO mice (n = 9) compared with WT mice (n = 11). *P < 0.05, **P < 0.01, significant difference between WT and Fxr2 KO (two‐tailed Student's t‐test: GluK5: t ₁₈ = 0.87, P = 0.394; PSD‐95: t ₁₈ = 2.29, P < 0.05; GluN2B: t ₁₈ = 3.09, P < 0.01; mGluR5: t ₁₈ = 3.74, P < 0.01). Quantified bands are highlighted with an asterisk or squared bracket. Data are presented as mean ± SEM.
2. Bar plot showing the mRNA levels of Grin2b, Grm5 and Dlg4, normalized for the average of Vinculin and Gusb mRNA levels in WT (n = 5) and Fxr2 KO (n = 5) hippocampi (two‐tailed Student's t‐test, P = n.s.). Data are presented as mean ± SEM.