Figure 1.
Identification of eight distinct non-hematopoietic cell populations
(A) HSPCs transduced with either thrombopoietin (ThPO) cDNA or control cDNA (EV) were transplanted (tx) at 0 weeks; n = 4 mice per time point and condition. Endpoint: 5 weeks (pre-fibrosis) and 10 weeks (fibrosis). Representative HE and reticulin staining. Scale bar, 50 μm.
(B) Hemoglobin (Hb) and platelet counts over time (mean ± SEM). Two-way-ANOVA with post hoc pairwise t test was used.
(C) UMAP of the non-hematopoietic BM niche identified by unsupervised clustering (n = 2,294 cells).
(D) Average gene expression of top marker genes within major cell populations (left panel). Feature plot of top markers in UMAP (right panel) is shown. Wilcoxon rank-sum test, p < 0.01). A full list of marker genes and corresponding GO terms is in Table S2.
(E) Heatmap of different MSC marker genes. Wilcoxon rank-sum test, p < 0.01.
(F) Venn diagram of the four identified MSC populations. Shared markers are highlighted in intersections.
(G) Heatmap SCP marker genes. Wilcoxon rank-sum test, p < 0.01.